Permselectivity Replication of Artificial Glomerular Basement Membranes in Nanoporous Collagen Multilayers
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چکیده
منابع مشابه
Immunohistochmistry Study of Collagen IV Changes in Glomerular Basement Memebrane During Fetal and Postnatal Periods of Balb/c Mice
Purpose: In this investigation specific antibody type IV collagen has been used in light microscopy to study development of BMG of the embryonic and postnatal mouse glomerular mesangium. Materials and Methods:20 female Balb/C mice were selected randomly and were kept under normal condition, finding vaginal plug was assumed as day zero of pregnancy. 12 pregnant mice were scarified by cervical di...
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Native and de novo synthesized heparan sulfate-proteoglycan (HS-PG) of basement membranes from isolated whole glomeruli were characterized. Sepharose CL-6B chromatograms of [35S]sulfate-labeled de novo synthesized HS-PG extracted from whole glomeruli indicated identical molecular weight characteristics to that isolated from purified basement membranes (Mr of intact HS-PG approximately equal to ...
متن کاملOn the methodology for measuring thickness of glomerular basement membranes.
Sir, We read with interest the multicentre Italian study by Frascà et al. [1] that confirms that a proportion of patients (6/51) with thin glomerular basement membrane disease (TBMD) have an underlying type IV collagen mutation that may not always be benign. In the ‘subjects and methods’ section they state that the measurements of glomerular basement membrane was performed by the method publish...
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Kidney glomerular basement membranes (GBMs) undergo laminin and type IV collagen isoform substitutions during glomerular development, which are believed to be required for maturation of the filtration barrier. Specifically, GBMs of earliest glomeruli contain laminin α1β1γ1 and collagen α1α2α1(IV), whereas mature glomeruli contain laminin α5β2γ1 and collagen α3α4α5(IV). Here, we used confocal mi...
متن کاملBovine Glomerular Basement Membrane
The collagenous domain of bovine glomerular basement membrane was excised in soluble form by limited pepsin digestion and characterized. The domain amounts to approximately 25% of the whole membrane by weight. The constituent polypeptides were studied using several electrophoresis systems and amino acid analysis. The electrophoresis systems used were a combination of agarose and polyacrylamide...
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ژورنال
عنوان ژورنال: The Journal of Physical Chemistry Letters
سال: 2011
ISSN: 1948-7185
DOI: 10.1021/jz200880c